Effects of Clemastine Fumarate on Expression of Toll Like Receptor 4 in Lung Ischemia-Reperfusion
10.3969/j.issn.1006-5725.2016.18.013
- VernacularTitle:富马酸氯马斯汀在肺缺血／再灌注损伤中对TLR4表达的影响
- Author:
Ning LIU
;
Wei WANG
;
Rui ZHANG
;
Huirong HAN
- Publication Type:Journal Article
- Keywords:
Toll like receptor 4;
Clemastine fumarate;
Lung ischemia-reperfusion injury;
Lung protective
- From:
The Journal of Practical Medicine
2016;32(18):2988-2991
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression change of Toll Like Receptor 4 (TLR4) in lung ischemia-reperfusion injury in rabbits and the influence of clemastine fumarate on it. Methods Fifty rabbits were divided into five groups randomly (n=10): Sham (N+S), reperfusion for 2 hours (N+I1/R2), reperfusion for 4 hours (N+I1/R4), clemastine fumarate + reperfusion for 2 hours (F+I1/R2) and clemastine fumarate+reperfusion for 4 hours (F+I1/R4). The ischemia time in each group was 1 hour and normal saline was given respectively in groups of N+S , N+I1/R2 and N+I1/R4. Western blotting , RT-PCR and immunofluorescence were used to detect the expression of TLR4 in lung tissue , and the changes of ultrastructure in ischemia-reperfusion lung tissue were observed by electron microscope. Result The expression of TLR4 was elevated obviously in ischemia-reperfusion lung tissue (P<0.05), and there was positive correlation between the increased TLR4 level and reperfusion time (P<0.05), the swelled and thick-ridge mitochondria were observed in type II alveolar epithelial cells after LIRI (P<0.05); but clemastine fumarate inhibited the expression of TLR4 in lung tissue significantly caused by LIRI (P<0.05). And the mitochondria injury was reduced in the groups of clemastine fumarate. Conclusion TLR4 expression is elevated in lung tissue after LIRI; clemastine fumarate inhibits the expression of TLR4 caused by LIRI and protects the lung tissue from LIRI in rabbits.
