Expression of miR-200c in endometrial carcinoma and its correlation with ZEB2 protein
10.3969/j.issn.1006-5725.2016.18.032
- VernacularTitle:miR-200c在子宫内膜癌组织中的表达及与ZEB2蛋白的相关性
- Author:
Chun SU
;
Qiaoyun LI
;
Wei ZHANG
- Publication Type:Journal Article
- Keywords:
Endometrial carcinoma;
miR-200c;
ZEB2;
Correlation
- From:
The Journal of Practical Medicine
2016;32(18):3057-3059
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of miR-200c in endometrial carcinoma and its correlation with ZEB2 protein. Methods The expression levels of miR-200c and ZEB2 gene and protein in tissues were detected. The expression of miR-200c in endometrial carcinoma RL95-2 cell line was inhibited by using antisense oligonucleotides and its effect on cell invasiveness was tested. The expressions of ZEB2 gene and protein in cells were detected. Results The expression levels of miR-200c and ZEB2 mRNA and protein in endometrial tissues were significantly higher than those in adjacent tissues and control group (P < 0.05). The average penetrating number in antisense miR-200c transfected group was significantly less than negative control group and liposome group (P < 0.05). The expression levels of ZEB2 gene and protein in antisense miR-200c transfected group were lower than the negative control group and liposome group (P < 0.05). Conclusion The expression of mRNA-200c in endometrial carcinoma was high and it might be promoting tumor cell invasion and metastasis by regulating ZEB2.
