Effect of Aurora kinase inhibitors VX-680 on proliferation of undifferentiated thyroid carcinoma cell
10.3969/j.issn.1006-5725.2016.17.005
- VernacularTitle:Aurora激酶抑制剂VX-680对未分化甲状腺癌细胞增殖的影响
- Author:
Hada HEYUAN
;
Lin CHEN
;
Yong YANG
;
Hongxin YANG
- Publication Type:Journal Article
- Keywords:
Thyroid carcinoma;
VX-680;
Aurora-A;
Bcl-2;
Bax
- From:
The Journal of Practical Medicine
2016;32(17):2791-2794
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Aurora-A kinase inhibitors VX-680 on the proliferation of thyroid carcinoma cell and to study their molecular mechanism. Methods The undifferentiated thyroid carcinoma cells were divided into groups of VX-680 with different drug concentration. Drug effect was observed by MTT method to measure the changes of cell proliferation. The gene expression of Aurora-A , Bcl-2 and Bax were tested by immunofluorescence assay. Results MTT results showed cell proliferation had been suppressed obviously with the of concentration increase of drug and the duration of administration (P < 0.05). The immunofluorescence results of 48 h showed Aurora-A and Bcl-2 protein expression of 400 nmoL were lower than those of other groups (P < 0.05), and Bax protein expression was similar to that of other groups(P > 0.05). Conclusions Aurora-A and Bcl-2 protein expression of thyroid cancer are inhibited when VX-680 being used , at the same time , Bcl-2/Bax scale value loses balance to accelerate forming Bax/Bax homodimer. Thus cancer cell proliferation is blocked and cell apoptosis is induced. So VX-680 is a good target inhibitor for anaplasia thyroid cancer.
