Effect of Salvia miltiorrhiza injection on proliferation, apoptosis and expression of amino acid in human embryonic lung fibroblast cells
10.3969/j.issn.1006-5725.2016.17.006
- VernacularTitle:丹参注射液对人胚肺成纤维细胞增殖、凋亡及羟脯氨酸表达的影响
- Author:
Fei QU
;
Haiyun[ LIU
;
Yanru CUI
- Publication Type:Journal Article
- Keywords:
Salvia miltiorrhiza injection;
Human embryonic lung fibroblast cells;
Proliferation;
Apoptosis;
Hydroxyproline
- From:
The Journal of Practical Medicine
2016;32(17):2795-2799
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Salvia miltiorrhiza injection on proliferation , apoptosis and expression of hydroxyproline in human embryonic lung fibroblast cells. Methods TGF-β1 was administered to induce the proliferation in human embryonic lung fibroblast cells; the effect of Salvia miltiorrhiza injection on the number of human embryonic lung fibroblast cells was detected by MTT method; ki67 expression by immunocytochemical method;cell apoptosis by flow cytometry and the expression of hydroxyproline by colorimetry method. Results TGF-β1(0, 2.5, 5, 10, 20, 40 μg/L)could up-regulate cell number of human embryonic lung fibroblast cells in a dose-dependent manner , while the OD value of human embryo lung fibroblasts cells declined pretreated with Salvia miltiorrhiza injection in a dose-dependent manner and Salvia miltiorrhiza injection could induce the apoptosis and down regulated hydroxyproline expression in human embryo lung fibroblasts cells. The results of flow cytometry indicated that cell apoptosis increased after treated with Salvia miltiorrhiza injection when compared with TGF-β1 group (P < 0.05). Meanwhile, Salvia miltiorrhiza injection could down regulate the expression of hydroxyproline (P < 0.01). Conclusions Salvia miltiorrhiza injection can target human embryonic lung fibroblast cells , play a potent role in the airway remodeling through the promotion of its apoptosis and down regulate the expression of hydroxyproline.
