The influence of the toll-like receptor-4 signaling pathway on IFN-γ and PDGF-AB expressions in the synthesis and secretion of pulmonary artery smooth muscle cells of COPD rats
10.3969/j.issn.1006-5725.2016.20.019
- VernacularTitle:TLR-4信号通路对COPD大鼠肺动脉平滑肌细胞合成分泌IFN-γ、PDGF-AB的影响
- Author:
Pengyan WANG
;
Ming JIANG
;
Xuhui HAN
;
Changming WANG
;
Dan YANG
- Publication Type:Journal Article
- Keywords:
LPS;
TLR4 signaling pathway;
COPD;
PASMCs
- From:
The Journal of Practical Medicine
2016;32(20):3350-3353
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the function of the toll-like receptor-4 (TLR-4) signaling pathway in the synthesis and secretion of pulmonary artery smooth muscle cells of rats with COPD. Methods The primary pulmonary artery smooth muscle cells (PASMCs) of rats with COPD were digested, separated and purified. Then they were randomly divided into control group, LPS group, TLR4 inhibitor group (TAK242) and LPS + TLR4 inhibitor group. RT-PCR, Western blot were used to detect the expression level of TLR-4 and NF-κB among groups. The levels of IFN-γ and PDGF-AB in supernatant with PASMCs in each group were detected by ELISA. Results LPS increased the expression of TLR-4、 NF-κB and the levels of IFN-γ and PDGF-AB. The expression of TLR4, NF-κB and the levels of IFN-γ and PDGF-AB were significantly reduced after inhibiting the expression of TLR4(P < 0.05). Conclusion TLR-4 signaling pathway involved in the inflammatory response and pulmonary vascular remodeling which increased the synthesis and secretion of IFN-γ and PDGF-AB in PASMCs. It provides a theoretical approach for the early intervention of clinical with COPD.
