Effect of angiotensin Ⅱ on the expression of MIF in macrophages
10.3969/j.issn.1006-5725.2016.20.025
- VernacularTitle:血管紧张素Ⅱ对巨噬细胞移动抑制因子表达的影响
- Author:
Yanning WANG
;
Xuguang WANG
;
Min ZHANG
;
Ying HAN
;
Fan WU
- Publication Type:Journal Article
- Keywords:
Atherosclerosis;
Angiotensin Ⅱ;
MIF;
NF-κB
- From:
The Journal of Practical Medicine
2016;32(20):3372-3375
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether angiotensin Ⅱ (AngⅡ) can regulate the expression of MIF in macrophages via the NF-κB pathway. Methods Western Blot, real time RT-PCR and ELISA were used in the present study. Results Western blot result showed that the expression of NF-κBp65 gradually increased with the increase of the concentration of AngⅡ. Results of real time RT-PCR and ELISA revealed that MIF mRNA expression and the content of MIF were significantly higher in AngⅡ group than those in the control group. PDTC could reverse the effect of AngⅡ on MIF mRNA expression and MIF secretion in macrophages. Conclusion AngⅡ can promote MIF mRNA expression and MIF secretion in macrophages via the NF-κB signaling pathway.
