Molecular mechanism of osteoclast differentiation induced by staphylococcal lipoteichoic acid
10.3969/j.issn.1006-5725.2016.20.024
- VernacularTitle:金葡菌脂磷壁酸促进破骨细胞分化的分子机制
- Author:
Lirong REN
;
Hai WANG
;
Xiaoqing HE
;
Muguo SONG
;
Xueqiu CHEN
;
Yongqing XU
- Publication Type:Journal Article
- Keywords:
Osteomyelitis;
Staphylococcal lipoteichoic acid;
Osteoclast differentiation;
Molecular mechanism
- From:
The Journal of Practical Medicine
2016;32(20):3369-3372
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the molecular mechanism of osteoclast differentiation induced by staphylococcal lipoteichoic acid (LTA-sa). Methods Raw264.7 cells were treated with LTA-sa in a concentration of 200 ng/mL for 0, 5, 10, 20, 40, 60 min and 0, 1, 2, 3 days respectively, and the proteins in signaling pathways associated with osteoclast differentiation were measured with western blot. In addition, Raw264.7 cells were treated with different concentrations of LTA-sa (100, 200 and 400 ng/mL) and PBS for 0, 1, 2, 3 days, the expression of TNF-α, IL-1α and IL-6 was detected with Enzyme linked immunosorbent assay (ELISA). Results (1)Western blot showed that, under stimulation of LTA-sa, IκB-α decreased at 5 min and 10 min, while the phosphorylation of nuclear factor κB increased at 10 min . In addition , NFATc1 increased in 2 and 3 days gradually. The above results were statistically analyzed, and the difference was significant in statistics (P < 0.001). (2)ELISA showed that the expression of IL-6 increased in 2 and 3 days along with the increasing concentration and prolonging stimulation time of LTA-sa. Data were statistically analyzed, the difference was significant in statistics (P < 0.001). Conclusion LTA-sa promotes osteoclast differentiation through the NF-κB signaling pathway and the secretion of IL-6.
