Study on Polymorphism of Nontraumatic Osteonecrosis of Femoral Head in Blood Stasis
10.3969/j.issn.1005-5304.2016.11.005
- VernacularTitle:血瘀质非创伤性股骨头坏死基因多态性研究
- Author:
Shenghua LI
;
Mingwang ZHOU
;
Tiefeng GUO
;
Haiping LIU
;
Binglin YE
;
Chengxiang WANG
- Publication Type:Journal Article
- Keywords:
nontraumatic osteonecrosis of femoral head (NONFH);
ApoA1 gene-75bp/+83bp point;
TCM constitutions;
blood stasis
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2016;23(11):17-21
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the correlation between nontraumatic osteonecrosis of femoral head (NONFH) and ApoA1 polymorphism of blood stasis type.Methods Totally 93 cases of NONFH were selected as the case group, and 83 healthy volunteers were randomly selected as the control group. With TCM constitution questionnaire survey, the case group was screened out 32 cases of blood stasis NONFH type and 61 cases of non blood stasis NONFH type. In the case group and control group, the subjects took blood samples 2 mL, extracted DNA for PCR amplification, PCR products for DNA sequencing. G994T PAF-AH and rs9658282 gene NOS1 site polymorphism were detected for tatistical analysis.Results -75G/A gene AA ApoA1 genotype (OR: 2.578; 95%CI: 1.174-5.663;P=0.018) and A allele (OR: 1.726; 95%CI: 1.121-2.658;P=0.013) may be one of the risk factors of NONFH.Conclusion -75G/A gene ApoA1 may be related to the pathogenesis of NONFH. There was no correlation between the ApoA1 gene polymorphism of -75G/A gene and the pathogenesis of blood stasis NONFH. There was no correlation between the ApoA1 gene polymorphism of +83C/T gene and the pathogenesis of blood stasis NONFH.
