Ethanol inhibiting the NLRP3 inflammasome activation to protect coronary heart disease

VernacularTitle:乙醇通过抑制NLRP3炎性小体减轻冠状动脉粥样硬化
Author: Jing ZHOU ; Yingping JIANG ; Yiqiao ZHAO
Publication Type:Journal Article
Keywords: Coronary atherosclerosis; Ethanol; Interleukin-1α; NLRP3
From: The Journal of Practical Medicine 2016;32(19):3164-3167
CountryChina
Language:Chinese
Abstract: Objective To examine whether ethanol modulates the intracellular processes involved in the secretion of IL-1α,and then exert a protective effect against coronary heart disease. Methods THP-1 cells in human were cultured for 2-3 generations , and put in PMF for 72 h to induce THP-1 into macrophage. ELISA was applied to detect effects for secretion of IL-1α by LPS, cholesterol and ethanol. In the light of ELISA re-sults, western blot was applied to detect the effects of ethanol on caspase-1 and NLRP3. Results Compared with the control group, the secretion of IL-1α in LPS group and LPS + CHOL group increased. Compared withLPS + CHOL group, the concentration of IL -1α in LPS + CHOL + etha group significantly decrease(P < 0.01). The results of western blot showed that ethanol significantly inhibited caspase-1 and NLRP3 activation. Conclu-sion Ethanol can inhibit the NLRP3 inflammasome activation in macrophages , which may represent a biological pathway underlying the protective effect of moderate alcohol consumption on coronary heart disease.