Effects of Extracts of Liaoxuan Kaxifu Powders on Proliferation of HaCaT Induced by TNF-α
10.3969/j.issn.1005-5304.2016.11.016
- VernacularTitle:疗癣卡西甫散提取物对肿瘤坏死因子-α诱导角质形成细胞增殖的影响
- Author:
Xiaoming PENG
;
Li GAO
;
Shixia HUO
;
Mo ZHAO
;
Ming YAN
- Publication Type:Journal Article
- Keywords:
extracts ofLiaoxuan Kaxifu Powders;
TNF-α;
HaCaT;
proliferation;
IL-8;
ICAM-1
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2016;23(11):67-70
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effects of extracts ofLiaoxuan Kaxifu Powders (LE) on proliferation and transcription of IL-8 and ICAM-1 in HaCaT induced by TNF-α; To discuss its mechanism of action.Methods Cultured HaCaT was assigned into normal group, TNF-α group and low-, medium- and high-dose of LE group. Every group was induced by 40 ng/mL TNF-α except for normal group, and then LE groups were treated by different concentrations (8, 40, 200μg/mL) of LE for 48 h. The proliferation of HaCaT was evaluated by MTT and the apoptosis was detected by inverted fluorescence microscope. Levels of IL-8 and ICAM-1 in HaCaT were assessed by ELISA, and their mRNA expressions was detected by semi-quantitative RT-PCR.Results Compared with normal group, the absorbency of HaCaT and the contents and mRNA expressions of IL-8 and ICAM-1 increased in TNF-α group (P<0.05,P<0.01); compared with TNF-α group, LE of all dose groups could significantly inhibit the absorbency, decrease the contents and mRNA expressions of IL-8 and ICAM-1 (P<0.05,P<0.01).Conclusion LE is able to inhibit the proliferation of HaCaT induced by TNF-α, and the mechanism is probably related to promoting apoptosis and down-regulating the gene expressions of IL-8 and ICAM-1, and then maintaining the normal level of HaCaT.
