Impact of High Intensity Focused Ultrasound and N2O on SKVO3 cell

VernacularTitle:高强度聚焦超声联合N2O对SKVO3细胞影响的实验研究
Author: Mingyuan PAN ; Zedan TIAN ; Fang LIU ; Qi WANG
Publication Type:Journal Article
Keywords: Nitrous oxide; High Intensity Focused Ultrasound; Cell apoptosis
From: The Journal of Practical Medicine 2016;32(18):2971-2973
CountryChina
Language:Chinese
Abstract: Objective To explore the effect of N2O with High Intensity Focused Ultrasound (HIFU) on SKVO3 through observing the influence of HIFU and N2O, separated and combined, on SKVO3. Methods The cultured SKVO3 cells were divided into 4 group: control group: without any treatment; N2O group: blowed by N2O 60 minutes; HIFU group: irradiated by HIFU (250W, 5s) and N2O-HIFU group blowed by N2O 60 minutes and irradiated by HIFU (250W, 5s). Flow cytometric method and Annexin V-FITC/PI binding assay were applied to detect cell death and apoptosis, and western blot test to detect Cytochrome C, Capase 3, Bax, Bcl-2 and GAPDH. Results The cell death and apoptosis in N2O-HIFU group were significant higher than those in other groups(P < 0.05). The results of western blot showed that in N2O-HIFU group, CytochromeC, Caspase 3 and Bax increased remarkably, but Bcl-2 significantly suppressed. Conclusion HIFU combininh with N2O could enhance the killing of SKVO3 cell.