Effects of liraglutide on the expression of NF kappa-B and inflammatory factor in kidney of insulin resistance rats
10.3969/j.issn.1006-5725.2016.18.016
- VernacularTitle:利拉鲁肽对胰岛素抵抗大鼠肾脏NF－κB及炎症因子表达的影响
- Author:
Xiaojing WU
;
Guicai HU
;
Yu GAO
;
Minghao WU
;
Jian LIU
- Publication Type:Journal Article
- Keywords:
Insulin resistance;
Liraglutide;
Nuclear factor-kappa B;
tumor necrosis factor-alpha;
Interleukin-6
- From:
The Journal of Practical Medicine
2016;32(18):3000-3003
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of liraglutide on the expression of NF-kappa B (NF-κB), tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) in renal tissue of insulin resistance rats. Method The rats were divided into 4 groups, control group ,high fat group ,liraglutide low dose group [100 μg/(kg· d)], liraglutide high dose group[100 μg/(kg·d)]. Detection of serum creatinine and urea nitrogen by automatic biochemical analyzer, and 24 h urine micro albumin was determined by immunoassay. The protein concentration of serum NF-κB,TNF-α and IL-6 was measured by ELISA. The expression of NF-κB,TNF-α and IL-6 mRNA in renal tissues was determined by RT-PCR. Results Compared with the control group, the serum creatinine, blood urea nitrogen,urinary protein, and the expression of NF-κB, TNF-α and IL-6 were significantly higher in high fat group, respectively (P < 0.05). The glomerular basement membrance thickening and foot process fusion were seen under electron microscope. Liraglutide high dose intervention can significantly reduce these changes. Conclusions Liraglutide can inhibit the expression of NF-κB,TNF-α and IL-6 in renal tissues of high fat induced insulin resistance rats,in a concentration dependent manner, which possibly improve renal function.
