Correlation between the changes of peripheral blood myeloid-derived suppressor cells and the level of serum nitric oxide in hepatocarcinoma mice
10.3760/cma.j.issn.1006-9801.2016.10.003
- VernacularTitle:肝癌小鼠外周血髓系抑制性细胞比例与血清一氧化氮水平关系的研究
- Author:
Xiaoqin XU
;
Sulian ZHAO
- Publication Type:Journal Article
- Keywords:
Liver neoplasms;
Myeloid-derived suppressor cells;
Nitric oxide;
Models,animal
- From:
Cancer Research and Clinic
2016;28(10):659-663
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the changes of myeloid-derived suppressor cells (MDSCs) in peripheral blood and serum nitric oxide in tumor-bearing mice, and to explore their correlation and functions in tumor progression. Methods Orthotopic transplantation tumor model of hepatocarcinoma in mice was established. At the 7th, 14th, 21st day after tumor implantation, the proportion of peripheral blood MDSCs was tested by flow cytometry, and serum nitric oxide was detected using the method of nitric acid reductase. Results The proportion of peripheral blood MDSCs at the 7th, 14th, 21st day after tumor implantation in tumor-bearing mice was significantly higher than that in normal mice [(8.65±1.01)%, (14.20±2.52)%, (27.51± 2.98) % vs. (2.51 ±0.44) %, P<0.05]. The level of nitric oxide at the 7th, 14th, 21st day after tumor implantation in tumor-bearing mice was significantly lower than that in normal mice [(72.02 ±5.05) μmol/L, (94.70 ±3.34) μmol/L, (78.90 ± 8.19) μmol/L vs. (57.03 ±16.94) μmol/L, P<0.05]. The level of serum nitric oxide was positively associated with the proportion of MDSCs (r=0.689, P<0.01). Conclusion The proportion of peripheral blood MDSCs and the level of serum nitric oxide in mice are increased with the tumor progression, and there is a positive correlation between them.
