Effect of niaoduqing in combination with telmisartan on the diabetic nephropathy in rats
10.3969/j.issn.1006-5725.2016.17.004
- VernacularTitle:尿毒清联合替米沙坦对糖尿病大鼠肾脏保护作用及机制
- Author:
Qizhe WU
;
Xiaoyan WU
;
Dongqing ZHA
- Publication Type:Journal Article
- Keywords:
Uremic Clearance;
Telmisartan;
Diabetic nephropathy rat;
Dimerization
- From:
The Journal of Practical Medicine
2016;32(17):2787-2790
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Niaoduqing combined with Telmisartan on the diabetic nephropathy in rats. Methods Forty female Wistar rats were randomly divided into the normal control group ( NC ) , the diabetic nephropathy in the control group ( DM ) , the uremic clearance group ( ND ) , and the combination group (LH). Rats in the DM, ND, LH groups were used to establish the diabetic nephropathy model. Rats in each group received the corresponding drugs. Twelve weeks later, the interactions among angiotensin receptor and adiponectin receptor were detected by co-immunoprecipitation assay. Western blot was performed to detect the expression of MCP-1, TGF-βto reveal the injury of the renal tubular interstitial. Results (1)Exogenous co-immunoprecipitation assay indicated that AdipoR1 and AT1, AT2 can form dimerization. Levels of AdipoR1-AT1, AdipoR2-AT2 dimerization were incresaed in the DM group compared with those in the NC group, but were decreased in the ND group and the LH group. (2)MCP-1 and TGF-βprotein expression were increased in the DM group compared with the normal control group (P < 0.05), but were decreased in the ND group and the LH group (P<0.05, respectively). Conclusion Niaoduqing combined with Telmisartan can significantly inhibit the expression of MCP-1 and TGF-βto relieve the renal tubular interstitial injury in rats with diabetic nephropathy. This protective mechanism may associated with the decrease of the dimerizations of AdipoRs and ATs.
