The Effect of Ulinastatin on Endoplasmic Reticulum Stress Associated Molecules Expressions in Rat Cerebral Cortex After Cerebral Ischemia Reperfusion
10.3969/j.issn.1671-332X.2016.10.005
- VernacularTitle:乌司他丁对大鼠脑缺血再灌注后大脑皮质内质网应激相关分子表达的影响
- Author:
Yuping DENG
;
Huangsen HUANG
;
Xiaoqiang LIAN
- Publication Type:Journal Article
- Keywords:
Ulinastatin;
endoplasmic reticulum stress;
ischemia reperfusion
- From:
Modern Hospital
2016;16(10):1423-1426
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effects of ulinastatin on express of GRP 78、CHOP and caspase-12, the molecules related to endoplasmic reticulum stress (ERS),after ischemia reperfusion injury in rats.Methods Ninety rats were equally randomized into 3 groups(n=30): Sham group (S group,n=30), Ischemia -reperfusion group (I/R group, n=30), Ulinastatin group (U group, n=30).Focal transient cerebral ischemia model was established with intralu-minal occlusion fo left meddle cerebral artery .Made through 2 hours of temporary middle cerebral artery occlusion , followed with 24 hours of reperfusion .The pathological results were investigated by HE staining and the cerebral injury situation was e -valuated by neurological deficit scores .Infract volume was measured by TTC staining , apoptosis was detected by TdT -medi-ated dUTP and nick end labeling (TUNEL), and expression of GRP78, CHOP and caspase -12 were meastured by western blot.Results Compared with the S group , the number of apoptotic cells were significantly increase in I /R group and U group (P<0.05);infarct volume and expression of GRP -78, CHOP and caspase-12 were significantly increased in I/R group and U group (P<0.05).The infarct volume and the number of apoptotic cells were significantly less in U group than in I/R group ( P<0.05 ) .GRP78 expression was higher in U group than in I/R group ( P<0.05 ) , however CHOP and caspase-12 expression was less in U group than in I/R group (P<0.05).Conclusion Ulinastatin has a protective effect on cerebral ischemia reperfusion injury , which may related to increased GRP 78, decreased CHOP and caspase -12 expres-sion and to inhibition of the ERS -induced apoptosis pathway .
