Comparison of Simultaneous Determination of Alkaloids in Bile Processed Coptidis Rhizoma by QAMS and External Standard Method

VernacularTitle:一测多评法与外标法测定胆黄连中生物碱类成分含量比较
Author: Jing WANG ; Yue CHENG ; Zimin YUAN
Publication Type:Journal Article
Keywords: QAMS; external standard method; bile processed Coptidis Rhizoma; alkaloids; relative correction factors
From: Chinese Journal of Information on Traditional Chinese Medicine 2016;23(11):78-80
CountryChina
Language:Chinese
Abstract: Objective To establish QAMS method to determine the contents of three alkaloids in bile processed Coptidis Rhizoma; To compare the results of QAMS with those from external standard method; To prove the feasibility of QAMS.Methods An HPLC method was developed. Berberine hydrochloride was selected as the internal reference substance. 2 relative correction factors (RCF) of berberine hydrochloride to palmatine hydrochloride and to jatrorrhizine hydrochloride were established. Obtained RCFs were used to conduct content calculation (calculated value) to complete QAMS method. At the same time, the contents (measured value) of the three components were also determined by external standard method. Calculated value and measured value were compared.Results The analysis results showed that there was no significant difference between the calculated values and the measured values of the three alkaloids in 10 batches of bile processed Coptidis Rhizoma.Conclusion The QAMS method can be applied in the determination of alkaloids in bile processed Coptidis Rhizoma.