Separation of Ephedrine and Pseudoephedrine by Capillary Electrophoresis
10.3969/j.issn.1005-5304.2016.11.022
- VernacularTitle:麻黄碱和伪麻黄碱的毛细管电泳分离研究
- Author:
Shanshan QIAO
;
Xiongfei WANG
;
Yuezhu DING
;
Mengting HE
;
Huan YANG
;
Ruijuan YUAN
- Publication Type:Journal Article
- Keywords:
ephedrine;
pseudoephedrine;
capillary electrophoresis;
cyclodextrin
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2016;23(11):90-93
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a capillary electrophoresis method to separate ephedrine and psedudoephedrine. Methods RM-β-CD and HP-β-CD were set as additives. A capillary electrophoresis method was set up. The effects of types and concentrations of additives, the concentrations and pH values of buffered solution, running voltage and organic solvent on the separation of ephedrine and psedudoephedrine were investigated.Results Ephedrine and pseudoephedrine could be successfully separated by using either RM-β-CD or HP-β-CD as additives. When RM-β-CD was used as additive, the best separation conditions were as follows: separation voltage 10 kV, 25 mmol/L Tris-H3PO4 (pH 2.42), 20 mg/mL of RM-β-CD. Under the conditions, the resolution of ephedrine and pseudoephedrine was 1.56 and they were separated successfully within 13 min. When HP-β-CD was used as additive, the best separation conditions were as follows: separation voltage 10 kV, 25 mmol/L Tris-H3PO4 (pH 3.00), 50 mg/mL of HP-β-CD. Under the conditions, the resolution of ephedrine and pseudoephedrine was 2.73 and they were separated successfully within 15 min.ConclusionThis method is reliable, rapid and repeatable. It can be used as separation determination method for ephedrine and pseudoephedrine.
