Expression of Wnt5a gene in breast cancer cell line MCF-7 and its relationship with E-cadherin or vimentin
10.3760/cma.j.issn.1006-9801.2016.10.001
- VernacularTitle:Wnt5a基因在乳腺癌细胞株MCF-7中的表达及其与E-钙黏蛋白、波形蛋白基因的关系
- Author:
Xia QIU
;
Guorong YANG
;
Li LIU
;
Yong HUANG
;
Ruolan ZENG
;
Xiaoyi YAO
;
Jing SHI
;
Zhifo WU
;
Ling WANG
;
Gui LIU
;
Xinjia CAI
;
Junhui HUANG
- Publication Type:Journal Article
- Keywords:
Breast neoplasms;
Wnt5a;
E-cadherin;
Vimentin;
Epithelial-mesenchymal transition
- From:
Cancer Research and Clinic
2016;28(10):649-653
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between Wnt5a gene and E-cadherin or vimentin gene in breast cancer cell line MCF-7. Methods RT-PCR was used to detect the mRNA expression of Wnt5a, E-cadherin and vimentin in breast cancer MCF-7 cells and the normal human mammary epithelial cell line MCF-10A, respectively, and their correlation was analyzed. Results The mRNA expression levels of Wnt5a and E-cadherin in cell line MCF-7 were significantly lower than those in cell line MCF-10A [(16.93± 2.97)%vs. (27.47±2.76) %, (12.97±1.35) % vs. (20.43±2.60) %, both P<0.05]. The mRNA expression level of vimentin in cell line MCF-7 was significantly higher than that in cell line MCF-10A [(16.53±0.85)%(6.33± 2.08) %, P<0.05 ]. In cell line MCF-7, the expression of Wnt5a was positively related to E-cadherin (г=0.997, P<0.05), but it was negatively related to vimentin (г=-0.998, P<0.05). Conclusions The expression of Wnt5a in human breast cancer cell line MCF-7 is significantly lower than that in cell line MCF-10A, which indicates that Wnt5a is a cancer suppressor gene in breast cancer. The expression of Wnt5a in cell line MCF-7 is positively related with E-cadherin, and it is negatively related with vimentin. Wnt5a may cause invasion and metastasis of breast cancer cell through the breast epithelial mesenchymal transitions.
