Metabolomic study on preventive effect of Aqueous extract and Volatile oil of Dalbergia Odorifera on myocardial ischemia/reperfusion injury in rats
10.3969/j.issn.1001-1978.2016.10.010
- VernacularTitle:降香水提物和挥发油对心肌缺血/再灌注损伤大鼠预防作用的代谢组学研究
- Author:
Fei MU
;
Jialin DUAN
;
Haixu BIAN
;
Rui LIN
;
Peijin SHANG
;
Zhihui ZHU
;
Aidong WEN
;
Miaomiao XI
- Publication Type:Journal Article
- Keywords:
metabonomics;
GC-TOF-MS;
MI/R inju-ry;
DOA;
DOO;
energy metabolism
- From:
Chinese Pharmacological Bulletin
2016;32(10):1377-1382
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the changes of serum metabolism after the treatment of DOA and DOO on myocardial ischemia reperfusion ( MI/R ) injury in rats, and to explore the pathogenesis of MI/R injury and drug action mechanism. Method The serum samples of Sham group, Model group, DOA group and DOO group of rats were acquired, gas phase time of flight mass spectrometry ( GC-TOF-MS) was applied to analyze the metabolic profiles of the samples. After da-ta preprocessing, they were processed into SIMCA 14. 1 software for multivariate statistical analysis. Results By principal components analysis ( PCA ) , partial least squares analysis ( PLS-DA) and orthogonal partial least squares analysis ( OPLS-DA ) , the Model group and Sham group were obviously separated, the drug in-tervention group and Model group were separated and close to Sham group. The therapeutic effect of DOO and DOA on MI/R injury in rats was proved. The ex-perimental results identified 13 endogenous biomark-ers, which were related to the glucose metabolism,lipid metabolism and amino acid metabolism pathway. Con-clusion DOA and DOO may protect the MI/R injured rats by regulating the glucose metabolism, lipid metab-olism and amino acid metabolism pathway.
