Evaluation of regulational function of ingredients from hot herbs on TRPV1 channel based on 7900 PCR instrument
10.3969/j.issn.1001-1978.2016.10.013
- VernacularTitle:基于荧光定量PCR评测热性中药成分对TRPV1通道功能的影响
- Author:
Haiyu ZHOU
;
Li DAI
;
Defeng WANG
;
Yifei DAI
;
Weiwei ZHOU
;
Jing MENG
;
Feng SUI
;
Hairu HUO
- Publication Type:Journal Article
- Keywords:
ingredients from hot-nature herbs;
dorsal root ganglion neurons;
TRPV1 channels;
7900 PCR in-strument;
thermo-sensational function;
energy metabo-lism
- From:
Chinese Pharmacological Bulletin
2016;32(10):1395-1398
- CountryChina
- Language:Chinese
-
Abstract:
Aim To further study the molecular mecha-nism of the herbs with hot nature on the regulational action on TRPV1 channel based on the 7900 Real-time PCR instrument. Methods 7900 PCR instrument was applied to detect the intracellular flurescence of TRPV1 channel in the dorsal root ganglions ( DRG ) neurons and the effect on the TRPV1 ’ s thermo-sensational functions of the selected 11 ingredients from hot herbs was explored. Results TRPV1 channels could be ac-tivated by gradually elevated temperature; the activa-tion process could be blocked by the TRPV1 specific blocking agent capsaizepine. Most of the ingredients from hot-nature herbs had the potential to up-regualate TRPV1 channel function. Conclusions The estab-lished TRPV1 channel detection system based on PCR instrument is suitable for the analysis of regulational functions of drugs on the heat-activated TRPV1 chan-nel;the functions of hot herbs may be related to the up-regualtional effects of its active ingredients on the TRPV1 channel which will further up-regulate energy metabolism.
