Change in expression of WNK1 in spinal cord of a rat model with bone cancer pain
10.3969/j.issn.1001-1978.2016.10.021
- VernacularTitle:骨癌痛大鼠脊髓中WNK1激酶表达的变化
- Author:
Mengwei SHEN
;
Xiaobo QIAN
;
Jianling GAO
;
Lina WANG
;
Xiaowen MENG
;
Jianping YANG
- Publication Type:Journal Article
- Keywords:
bone cancer;
pain;
WNK1;
spinal cord;
NKCC1;
KCC2
- From:
Chinese Pharmacological Bulletin
2016;32(10):1442-1445
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the changes in the ex-pression of WNK1 in spinal cord of a rat model with bone cancer pain. Methods Female SD rats, weig-hing 170 ~200 g, were randomly divided into three groups:normal control group (group C, n=3), sham operation group ( group S, n =3 ) and bone cancer pain group ( group BCP, n =24 ) . Group C was not given any treatment, and group S was injected into the bone marrow of left tibia with 5 μl PBS solution while group BCP with 5 μl WALKER 256 mammary gland cancer cell suspension (approximately 1 × 105 cells). Mechanical paw withdrawal threshold ( MWT ) was measured at d1 before inoculation ( baseline) and d3, 6,9,10,11,12 after inoculation. Group S and C were sacrificed at d 12 while group BCP at d 3 ,6 ,9 ,12 after inoculation and spinal cord ( L4~6 ) were removed at different time points for detection of WNK1 mRNA ex-pression by qRT-PCR and WNK1 protein expression by Western blot. Results Compared with group C and S,group BCP’ s MWT started to decrease since d 3 ( P<0. 05 ) . The mRNA expression of WNK1 in spinal cord was up-regulated(P<0. 05) from d 3 showing an increasing trend over time until d 9 ( peak ) and then down-regulated to d 12 ( P >0. 05 ) while the protein expression upregulated since d6 and also showed an in-creasing trend to d 12 ( P<0. 01 ) . Conclusion The expression of WNK1 in spinal cord of a rat model with bone cancer pain increased abnormally, which may be involved in the occurrence and maintenance of a rat model with bone cancer pain.
