Characteristic and clinical significance of DNA methyltransferase 3B overexpression in endometrial carcinoma
10.3969/j.issn.1671-167X.2016.05.007
- VernacularTitle:子宫内膜癌中 DNA 甲基转移酶3B 的表达特点与临床意义
- Author:
Ying DONG
;
Mei ZHOU
;
Xiaojun BA
;
Jingwen SI
;
Wenting LI
;
Ying WANG
;
Dong LI
;
Ting LI
- Publication Type:Journal Article
- Keywords:
Endometrial neoplasms;
DNA methyltransferase 3B;
Methylation;
Receptors,estrogen;
Receptor,progesterone
- From:
Journal of Peking University(Health Sciences)
2016;48(5):788-794
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To determine the clinicopathological significance of the DNA methyltransferase 3B (DNMT3B)overexpression in endometrial carcinomas and to evaluate its correlation with hormone re-ceptor status.Methods:Immunohistochemistry was performed to assess the expression of DNMT3B and hormone receptors in 104 endometrial carcinomas.Results:DNMT3B overexpression occurred frequently in endometrioid carcinoma (EC,54.8%)more than in nonendometrioid carcinoma (NEC,30.0%) with statistical significance (P =0.028).Furthermore,there was a trend that EC with worse clinico-pathological variables and shorter survival had a higher DNMT3B expression,and the correlation between DNMT3B and tumor grade reached statistical significance (P =0.019).A negative correlation between DNMT3B and estrogen receptor (ER)or progesterone receptor (PR)expression was found in EC. NMT3B overexpression occurred frequently in the ER or PR negative subgroups (78.9%,86.7%)more than in the positive subgroups (47.7%,47.8%)with statistical significance (P =0.016,P =0.006). In addition,the DNMT3B overexpression increased in tumors with both ER and PR negative expression (92.9%,P =0.002).However,no such correlation was found in NEC (P >0.05).Sequence analyses demonstrated multiple ER and PR binding sites in the promoter regions of DNMT3B gene.Conclusion:This study showed that the expression of DNMT3B in EC and NEC was different.DNMT3B overexpres-sion in EC was associated with the worse clinicopathological variables and might have predictive value. The methylation status of EC and NEC maybe different.In addition,in EC,DNMT3B overexpression negatively correlated with ER or PR expression.In NEC,the correlation between DNMT3B and ER or PR status was not present.
