Correlation of Breast Cancer Cells Invasive and Stathmin Gene Expression
10.3969/j.issn.1671-7414.2016.05.004
- VernacularTitle:Stathmin表达水平与乳腺癌细胞侵袭能力相关性研究
- Author:
Ming YANG
;
Fang LIN
;
Ting HE
;
Lin WANG
;
Ke DONG
;
Huizhong ZHANG
- Publication Type:Journal Article
- Keywords:
Stathmin;
breast cancer;
aggressive
- From:
Journal of Modern Laboratory Medicine
2016;31(5):14-17
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship between the Stathmin gene expression in breast cancer cells MDA-MB-231, MCF-7 and the biological behaviours such as cell growth,adhesion and invasion,and provide experimental basis of breast cancer metastasis for further study.Methods Used RT-PCR and Western Blot methods to detect the Stathmin gene expres-sion levels in MDA-MB-231 and MCF-7 cells,and in the mean while to test the MDA-MB-231 and MCF-7 cell growth,adhe-sion,invasion ability by CCK-8 cell proliferation experiments,cell adhesion experiments,cell invasion experiments,then, analyed the relationship of Stathmin gene expression and cell growth,adhesion,invasion ability.Results Over-expression levels of Stathmin gene were observed both in the MDA-MB-231 and MCF-7 cells (F=10.173,P<0.05),and furthermore, the expression levels of Stathmin gene in MDA-MB-231 cells was higher than in MCF-7 cells (t=4.562,P<0.05).While, the growth,adhesion and invasion ability of the MDA-MB-231 cells was higher than that of MCF-7 cells(P<0.05).Conclu-sion The higher level of Stathmin gene expression,the stronger breast cancer cells had ability of growth,invasion,and ad-hesive.The Stathmin gene expression levels was closely correlated with breast cancer cell invasive.
