Sequence Analysis and Identification of A Novel HLA-A* 2 4∶3 2 7 Allele Been Designated by the HLA Nomenclature Committee of WHO
10.3969/j.issn.1671-7414.2016.05.005
- VernacularTitle:WHO HLA命名委员会命名的新等位基因 HLA-A*24∶327序列分析及确认
- Author:
Tianju WANG
;
Liping CHEN
;
Xiaofang WANG
;
Manni WANG
;
Jun QI
- Publication Type:Journal Article
- Keywords:
human leukocyte antigen;
sequence based typing;
group-specific sequencing primer;
novel allele
- From:
Journal of Modern Laboratory Medicine
2016;31(5):18-22
- CountryChina
- Language:Chinese
-
Abstract:
Objective To do analysis of sequence and identify a novel HLA-A allele in Chinese hematopoietic stem cell do-nors.Methods A rare HLA-A allele was initially detected by Luminex PCR-SSO typing,then the sample was sequenced by sequence-based typing (SBT)and the group-specific sequencing primer (GSSP)to confirm the mutation allele and locus.Re-sults The sequence of the sample results showed that the allele compared with the highest homologous allele HLA-A*24∶02∶01∶01 was the difference in the exon 3 at position 544 G>A,resulting in an amino acid sequence of HLA-A*24∶02∶01∶01 at position 158 change Ala to Thr.Conclusion This allele is a new HLA-A allele and has been designated as HLA-A*24∶327 by the HLA Nomenclature Committee of World Health Organization (WHO).
