Affection of CD2 6/DPP4 on CD4+T Cells and Relative Cytokines in Patients with Crytococcal Meningitis and Its Clinical Significance
10.3969/j.issn.1671-7414.2016.05.010
- VernacularTitle:CD26／DPP4对隐球菌脑膜炎患者CD4＋T细胞及相关细胞因子的影响与临床意义
- Author:
Tengda LI
;
Shuping LONG
;
Guixia XU
;
Yun LIU
;
Weiwei ZHANG
;
Cheng QIAN
;
Yuanlan HUANG
;
Qin QIN
;
Sunxiao CHEN
;
Anmei DENG
- Publication Type:Journal Article
- Keywords:
Crytococcal Meningitis;
CD2 6/DPP4;
interleukin-1 7;
interleukin-4;
interferon-γ
- From:
Journal of Modern Laboratory Medicine
2016;31(5):38-41
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the affection and clinical significance of CD26/DPP4 on CD4+T cells and its cytokines in patients withCrytococcalMeningitis.Methods Peripheral blood was collected from 36 patients diagnosed withCrytococcal Meningitis in Changhai Hospital and Changzheng Hospital,Shanghai from August,2011 to December,2015,meanwhile 36 health controls’was also acquired.Peripheral blood mononuclear cell (PBMC)was separated by density gradient centrifuga-tion,CD26+CD4+T and CD26-CD4+T cell groups were classified by Flow Cytometry,the expression level of cytokines was tested by reverse transcriptase-polymerase chain reaction (RT-PCR).The correlation between DPP4 activity,CD26+CD4+T (%)and APACHE II score,IL-17,TNF-α,IL-4,IFN-γwas measured by Pearson coefficient.Results CD26+CD4+T(%)between experimental and control groups was 13.35±3.83 vs 8.39±2.14 (t=6.78,P<0.000 1).DPP4 activity was 50.89±17.21 mU/ml vs 73.83±20.24 mU/ml (t=5.18,P<0.000 1),with statistically significant differences.In ex-perimental groups,CD26+CD4+T (%)was positively related with APACHE II score,IL-17,TNF-α(r=0.431,0.564, 0.688,P=0.003 8,0.001,0.004 6).DPP4 activity was negatively interrelated with APACHE II score,IL-17,TNF-α,IFN-γ(r=-0.544,-0.489,-0.678,-0.734;P<0.001).Conclusion CD26/DPP4 may be involved in the pathogenesis of Crytococcal Meningitis through regulation of Th subgroups,and it was the potential therapeutic target and the predicted marker of the disease.
