Screening and Characterization of Human Phage Antibody to Permethrin
10.3969/j.issn.1671-7414.2016.05.012
- VernacularTitle:急性淋巴细胞白血病单链抗体（scFv）的筛选与鉴定
- Author:
Weina MA
;
Xuelin LIU
;
Hongbin SONG
;
Jianliang SHEN
;
Youzhang HUANG
;
Yi LIU
;
Dan XIANG
- Publication Type:Journal Article
- Keywords:
antibodies;
phage display technology;
acute lymphoblastic leukemia;
scFv
- From:
Journal of Modern Laboratory Medicine
2016;31(5):46-49
- CountryChina
- Language:Chinese
-
Abstract:
Objective To do screening acute lymphoblastic leukemia patients scFv antibody single chain variable region to cre-ate conditions for the expression and obtain further specificity of antibody fragments.Methods In this study,patients with newly diagnosed acute lymphoblastic leukemia serum as coating antigen using phage display technology,screening phage an-tibody specificity from the semi-synthetic human phage antibody libraries,the first to target the immune antigen-coated tab-let,phage library was added,so that with the target antigen-specific binding phage antibody was immobilized on plates immu-nization,could not be specifically bound phages were rinsed.The eluted specific binding phage,E.coli infection.Could get the specific antibody gene containing phagemid.Results After three “adsorption-elution-amplification”screening process,got stronger leukemia patient antigen-specific phage antibody variable region fragment and identification.Conclusion Got better strain affinity antibody fragments,to create the conditions for the next fragment expression,identification and clinical appli-cation.
