Cell death type of zebrafish lateral line hair cellsinduced by cisplatin
10.16066/j.1672-7002.2016.10.008
- VernacularTitle:顺铂引起斑马鱼侧线毛细胞凋亡的实验研究
- Author:
Xiaoxiao MI
;
Xiaowen KONG
;
Yuan LI
;
Junping SHI
- Publication Type:Journal Article
- Keywords:
Animals,Laboratory;
Cisplatin;
Hair Cell;
Apoptosis
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2016;23(10):585-589
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore cell death type of lateral line hair cellsinduced by cisplatin in zebrafish. METHODS Zebrafish larva were incubated in 1mM cisplatin solution for 6 hrs to induce about 90%lateral line hair cells loss. Time lapse imaging was used to detect the morphology of cisplatin-incubated hair cells in wildtypezebrafish pre-labelled by live dyes Bodipy TR C5-ceramide and Sytogreen 24. TUNEL assay and In situ anti-active Caspase-3 antibody staining were performed to detect nuclei fragmentation and Caspase-3 activity respectively. RESULTS Compared to control group, hair cells condensationand nuclei fragmentation (P<0.05) were detected in cisplatin-incubated group, and active Caspase-3 activity was also observed after cisplatin addition. CONCLUSION Cisplatinmay induced zebrafish lateral line hair cells loss by Caspase-3-dependent apoptotic pathway.
