The forensic application of Lefort aqua regia digestion method detecting diatom in rabbits′s organs(
10.13618/j.issn.1001-5728.2016.05.011
- VernacularTitle:勒福特王水消解法检验动物器官硅藻的法医学应用
- Author:
Huipin WANG
;
Yong CHEN
;
Jian ZHAO
;
Chao LIU
- Publication Type:Journal Article
- Keywords:
forensic pathology;
drowning;
Lefort aqua regia;
diatom
- From:
Chinese Journal of Forensic Medicine
2016;31(5):470-472
- CountryChina
- Language:Chinese
-
Abstract:
Objective The forensic application of Lefort aqua regia digestion method detecting diatom in rabbits’s organs. Methods Using the Lefort aqua regia digestion-membrane ifltrition-SEM observation method and the traditional acid digestion-centrifugation-light microscopy observation method to detect diatom of drowning or postmortem immersion rabbits’s organs(lung,liver,kidney), and make statistical analysis of diatom number, consuming time, digestive capability and ifne structure of diatoms. Results The diatom number of Lefort aqua regia digestion method was superior to conventional acid digestion method, with the difference being statistically signiifcant (P<0.05), as well as consuming time, but have undifferentiated of drowning grounp(P>0.05), and have a better observation effect. Conclusion The Lefort aqua regia digestion method for diatom extraction was helpful for diatom identiifcation., and useful in drowning test by conventional and economicly.