The identiifcation analysis of rhinoceros horns products based on DNA barcoding 12s rRNA
10.13618/j.issn.1001-5728.2016.05.012
- VernacularTitle:基于12s rRNA的犀牛角制品DNA条形码鉴定分析
- Author:
Yunxia CHEN
;
Haitao GUO
- Publication Type:Journal Article
- Keywords:
forensic biological evidence;
species identiifcation;
rhinoceros horns;
DNA barcoding;
12s rRNA
- From:
Chinese Journal of Forensic Medicine
2016;31(5):473-475
- CountryChina
- Language:Chinese
-
Abstract:
Objective Through the DNA barcoding 12s rRNA sequences alignment and analysis of several rhinoceros horns products involved in cases to analysis the application feasibility of 12s rRNA in the rhinoceros horns products’ species identification. Methods Use rhinoceros horns products in 3 cases as materials, total DNA were extracted with improved method, PCR ampliifcation the DNA barcoding. Results The alignment and analysis of sequences show that 12s rRNA could identify rhinoceros horns products at the species level. Conclusion The DNA barcoding 12s rRNA could be used as a new way to identify the rhinoceros horns products which can’t be identiifed with morphological characteristics, provide a reliable basis for the qualitative and sentencing of cases.
