Clinical significance of microrna-625 expression in esophageal squamous cell carcino-ma
10.3969/j.issn.1000-8179.2016.18.656
- VernacularTitle:MicroRNA-625在食管鳞癌中的表达及临床意义
- Author:
Shasha LIU
;
Dongli YUE
;
Xinfeng CHEN
;
Yu PING
;
Yi ZHANG
- Publication Type:Journal Article
- Keywords:
miR-625;
ESCC;
migration;
proliferation;
prognosis
- From:
Chinese Journal of Clinical Oncology
2016;43(18):825-829
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To analyze the correlation of miR-625 expression with clinicopathological characteristics in esophageal squa-mous cell carcinoma (ESCC) and to explore the effect of miR-625 on the migration and proliferation of ESCC cells. Methods:The expres-sion level of miR-625 was determined through real-time PCR in 86 paired human ESCC tissue specimens and tumor-adjacent normal esophageal tissue specimens, ESCC cell lines, and esophageal epithelial cell line. The associations of miR-625 expression with clinico-pathological characteristics and survival in ESCC patients were analyzed. Transwell and CCK-8 assays were performed to examine the effect of miR-625 expression on migration and proliferation of ESCC cells. Results:Compared with tumor-adjacent normal specimens, miR-625 was significantly downregulated in ESCC tissue specimens (P<0.05). MiR-625 expression was decreased in ESCC cell lines com-pared with human esophageal epithelial cell lines (P<0.05). Lower miR-625 expression was associated with poorer prognosis and sur-vival. The migration and proliferation abilities of ESCC cells were inhibited by miR-625 overexpression (P<0.05). Conclusion:MiR-625 acts as a tumor suppressor gene in the development and progression of ESCC, suggesting that miR-625 may serve as an efficient prog-nosis biomarker and a potential therapeutic target for ESCC.
