Prokaryotic expression and identification of rhoptry protein 38 of Toxoplas-ma gondii
10.16250/j.32.1374.2016060
- VernacularTitle:弓形虫棒状体蛋白ROP38的原核表达与鉴定
- Author:
Yong CUI
;
Jin LI
;
Hongfa WANG
;
Weixia ZHONG
;
Hui SUN
;
Guihua ZHAO
;
Kun YIN
;
Chao XU
;
Ting XIAO
;
Xiaoyu ZHANG
;
Hong YU
;
Xuefeng LIU
;
Gongzhen LIU
- Publication Type:Journal Article
- Keywords:
Toxoplasma gondii;
Rhoptry protein 38(ROP38);
Prokaryotic expression;
Parasitophorous vacuole(PV)
- From:
Chinese Journal of Schistosomiasis Control
2016;28(5):554-557
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the biological function of rhoptry protein 38(ROP38)of Toxoplasma gondii,and to iden?tify the reactogenicity of the recombinant protein(rROP38). Methods The ROP38 was amplified by RT?PCR from T. gondii RH strain,and was cloned into prokaryotic expression vector pET?28a(+). The recombinant plasmid was transformed into E. co?li BL21(DE3)competent cells. Then the rROP38 was analyzed by SDS?PAGE and identified by Western blot. Results SDS?PAGE showed that rROP38 was efficient expression with a molecular weight of about 43 kD. Western blot showed that rROP38 reacted with antibody of His tag or human positive antibody,which indicated that ROP38 had good reactogenicity and could be a serological diagnostic antigen. Conclusion The study successfully obtains the rROP38 of T. gondii with good reactogenicity.
