Impact of different degree pulpitis on cell proliferation and osteoblastic differentiation of dental pulp stem cell in Beagle immature premolars
10.3969/j.issn.1671-167X.2016.05.024
- VernacularTitle:不同炎症状态下犬年轻恒牙牙髓干细胞增殖及成骨分化能力的改变
- Author:
Long LING
;
Yuming ZHAO
;
Lihong GE
- Publication Type:Journal Article
- Keywords:
Pulpitis;
Mesenchymal stromal cells;
Osteoblasts;
Cell proliferation;
Cell differentiation
- From:
Journal of Peking University(Health Sciences)
2016;48(5):878-883
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To compare the proliferation and osteoblastic differentiation of dental pulp stem cell (DPSC)isolated from normal and inflamed pulps of different degrees in Beagle immature premolars, and provide evidence for the use of inflammatory DPSC (IDPSC).Methods:This study evaluated 14 Beagle’s young premolars (21 roots).In the experiment group,irreversible pulpitis was induced by pulp exposure and the inflamed pulps were extracted 2 weeks and 6 weeks after the pulp chamber opening.For the control group,normal pulps were extracted immediately after the exposure.HE staining and real-time PCR were performed to confirm the inflammation.The cells were isolated from the inflamed and normal pulps (IDPSC and DPSC).Cell proliferation and osteoblastic differentiation potentials of the two cells were compared.Results:Inflammation cells infiltration was observed in the inflamed pulps by HE stai-ning.The expression of inflammatory factor was much higher in the 6 week inflamed pulp.IDPSC had higher potential of cell proliferation and osteoblastic differentiation potentials.Furthermore,the osteoblas-tic differentiation potentials of IDPSC from 2 week inflamed pulp were higher than those from 6 week in-flamed pulp.Conclusion:The potential of cell proliferation and osteoblastic differentiation of DPSC was enhanced at early stage of irreversible pulpitis,and reduced at late stage in Beagle immature premolars.
