Preliminary study on infection status and gene types of Cryptosporidium among HIV/AIDS patients in Guangxi
10.16250/j.32.1374.2016038
- VernacularTitle:广西HIV/AIDS患者合并感染隐孢子虫现况及基因分型初步研究
- Author:
Zunfu WANG
;
Zhihua JIANG
;
Bingxue YU
;
Dongsheng ZHOU
;
Yuan LIN
;
Wenqian TANG
- Publication Type:Journal Article
- Keywords:
Cryptosporidium;
Infection;
HIV;
AIDS;
Nested-PCR;
Diarrhea;
Guangxi Zhuang Autonomous Region
- From:
Chinese Journal of Schistosomiasis Control
2016;28(5):550-553
- CountryChina
- Language:Chinese
-
Abstract:
Objective To understand the infection status and gene types of Cryptosporidium among HIV/AIDS patients in Guangxi Zhuang Autonomous Region. Methods The fecal samples were collected from 285 HIV/AIDS cases in Nanning,Gui?lin,Qinzhou,Baise,Hechi cities of Guangxi and 150 HIV negative persons in Nanning City. The modified acid?fast staining and nested?PCR based on 18S rRNA were employed to detect the infection status of Cryptosporidium. The nested PCR products were sequenced,and the homology searches and identification for the gene types of Cryptosporidium were done by DNAStar soft?ware. Results The infection rate of Cryptosporidium in HIV/AIDS patients was 0.70%(2/285),and the rate of those with chronic diarrhea was 6.67%(2/30),the latter was significantly higher than that of the HIV negative persons(0,0/150)(P=0.002). Both the two HIV/AIDS patients infected with Cryptosporidium were from Guilin City. By molecular identification,the Cryptosporidium strains which the above 2 patients were infected with were Cryptosporidium andersoni and Cryptosporidium hominis respectively. Conclusions Cryptosporidium co?infection can be found in HIV/AIDS patients in Guangxi. The geno?types of the infection strains include Cryptosporidium andersoni and Cryptosporidium hominis.
