Contents Determination of 4 Amino Acids in Pyrrosia lingua by RP-HPLC
10.6039/j.issn.1001-0408.2015.27.34
- VernacularTitle:RP-HPLC法同时测定石韦中4种氨基酸的含量
- Author:
Yi LONG
;
Wude YANG
;
Jihu YUAN
- Publication Type:Journal Article
- Keywords:
Pyrrosia lingua;
Amino acid;
RP-HPLC;
Content determination
- From:
China Pharmacy
2015;(27):3838-3840
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the contents determination of 4 amino acids in Pyrrosia lingua. METHODS:The samples were analyzed by RP-HPLC. It was performed on a column of Welchrom C18 with the mobile phase A of 0.1 mol/L So-dium acetate buffer(pH6.5)- acetonitrile(93∶7,V/V)and mobile phase B of acetonitrile-water(4∶1,V/V)(gradient elution)at the flow rate of 1.0 ml/min,detection wavelength was 254 nm,column temperature was 30 ℃ and the sample size was 5 μl. RE-SULTS:The linear range was 4.92-49.2 μg/ml for glutamate(r=0.999 1),3.168-31.68 μg/ml for glycine(r=0.999 9),2.6-26 μg/ml for alanine(r=0.999 4)and 2.88-28.8 μg/ml for leucine(r=0.999 9);RSDs of precision,stability and reproducibility tests were no more than 2.2%;average recoveries were 98.4%(RSD=2.2%,n=9),99.6%(RSD=2.5%,n=9),98.3%(RSD=2.2%,n=9)and 100.1%(RSD=2.3%,n=9),respectively. CONCLUSIONS:The method is simple,accurate and reproducible,and can be used for the contents determination of 4 amino acids in P. lingua.
