Simultaneous Determination of the Contents of 8 Ingredients of Shuanghuanglian Freeze-dried Powder in Pla-cental Perfusate
10.6039/j.issn.1001-0408.2015.27.28
- VernacularTitle:HPLC法同时测定胎盘灌流液中双黄连冻干粉中8种成分的含量
- Author:
Liying ZHAO
;
Dianrong SONG
- Publication Type:Journal Article
- Keywords:
Shuanghuanglian freeze-dried powder;
HPLC;
Placental perfusate;
Chlorogenic acid;
Forsythiaside B;
Forsythia-side A;
Baicalin;
Forsythin;
Luteolin;
Wogonoside;
Baicalein
- From:
China Pharmacy
2015;(27):3823-3825
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the simultaneous determination of the contents of Shuanghuanglian freeze-dried powder in placental perfusate. METHODS:HPLC was performed on the column of Agilent Zorbax-C18 with mobile phase of acetonitrile-1%formic acid aqueous solution(gradient elution)at flow rate of 1.0 ml/min,the internal standard was puera-rin,detection wavelength was 280 nm,column temperature was 25 ℃ and sample volume was 10 μl. RESULTS:There was a lin-ear range between linear ranges and peak area of 8 ingredients(r≥0.999 0);RSDs of within-day and inter-day precision tests were no more than 1.9%,repeatability tests was no more than 7.3%;average recoveries were in the range of 92.73%-112.37%(RSD=3.2%-8.2%,n=6);and average matrix effects were 90.33%-105.78%(RSD=3.2%-8.0%,n=6). CONCLUSIONS:The method is rapid,sensitive and specific,and can be used to the simultaneous determination of the contents of 8 ingredients of Shuan-ghuanglian freeze-dried powder in placental perfusate.
