Contents Determination of 4 Components in Yixinshu Capsule By Dual-wavelength HPLC
10.6039/j.issn.1001-0408.2015.27.36
- VernacularTitle:双波长HPLC法测定益心舒胶囊中4种成分的含量
- Author:
Shaozhi WANG
;
Xijie ZHANG
;
Lianhao FU
;
Xiaohong LIU
- Publication Type:Journal Article
- Keywords:
Dual-wavelength HPLC;
Yixinshu capsule;
Tanshinol;
Protocatechuic aldehyde;
Ferulic acid;
Salvianolic acid B
- From:
China Pharmacy
2015;(27):3844-3846
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the contents determination of tanshinol,protocatechuic aldehyde,ferulic ac-id and salvianolic acid B in Yixinshu capsule. METHODS:Dual-wavelength HPLC was performed on the column of Eclipse XDB-C18 with mobile phase of 0.5%phosphoric acid-methanol-acetonitrile(gradient elution)at the flow rate of 1.0 ml/min,the de-tection wavelength was 280 nm(tanshinol,protocatechuic aldehyde,salvianolic acid B)and 320 nm(ferulic acid),column tempera-ture was 30℃and volume was 10 μl. RESULTS:The linear range of tanshinol,protocatechuic aldehyde,ferulic acid and salvianolic acid B were respectively 9-144μg/ml(r=0.999 9),0.5-8μg/ml(r=0.999 9),0.65-10.4μg/ml(r=0.999 9)and 221.25-3 540μg/ml (r=0.999 9);RSDs of precision,stability and reproducibility tests were no more than 1.90%;the average recovery was respective-ly 100.8%(RSD=1.65%,n=9),100.1%(RSD=2.87%,n=9),100.1%(RSD=3.01%,n=9) and 99.4%(RSD=2.05%,n=9). CONCLUSIONS:The method is simple and reproducible,and can be used for the quality control of Yixinshu capsule.
