Study on the HPLC Fingerprint Chromatogram of Danzhen Headache Capsules
10.6039/j.issn.1001-0408.2015.27.43
- VernacularTitle:丹珍头痛胶囊的HPLC指纹图谱研究
- Author:
Yuxia ZHANG
;
Zhanquan ZAN
;
Tongxia WANG
;
Chunmei LI
;
Lanju JI
- Publication Type:Journal Article
- Keywords:
Danzhen headache capsules;
HPLC;
Fingerprint chromatogram;
Similarity
- From:
China Pharmacy
2015;(27):3864-3866
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the HPLC fingerprint chromatogram of Danzhen headache capsules. METHODS:HPLC method was adopted. The column was Dikma Diamonsil C18 with the mobile phase of methanol-acetonitrile-water(gradient elution) at the flow rate of 1.0 ml/min;the temperature was 40 ℃,the detection wavelength was 340 nm,the sample size was 20 μl and the detection time was 65 min. RESULTS:RSDs of precision,stability and reproducibility tests were no more than 0.23%;13 com-mon peaks were identified in 10 batches of Danzhen headache capsules and the similarity of all samples were higher than 0.90. CONCLUSIONS:The method is simple,accurate and reproducible,and can be used for the process stability evaluation and quali-ty control of Danzhen headache capsules.
