Content Determination of 5 Anthraquinones Constituents in Sanhuang Drop Pills by HPLC

VernacularTitle:HPLC法同时测定三黄滴丸中5种蒽醌类成分的含量
Author: Yanting WEI ; Jingyi ZHANG ; Huatan ZHANG ; Chunhua LI
Publication Type:Journal Article
Keywords: HPLC; Sanhuang drop pills; Aloeemodin; Rhein; Emodin; Chrysophanol; Physcion
From: China Pharmacy 2015;(27):3867-3869
CountryChina
Language:Chinese
Abstract: OBJECTIVE:To establish a method for the determining contents of 5 anthraquinones constituents in Sanhuang drop pills. METHODS:HPLC method was adopted. The column was Diamonsil C18 with the mobile phase of methanol-0.23% phosphoric acid(85∶15,V/V)at the flow rate of 1.0 ml/min,the detection wavelength was 440 nm and the volume temperature was room tem-perature. The sample size was 10 μl. RESULTS:The linear range was 0.070-0.700 μg for aloeemodin(r=0.999 9),0.646-6.460 μg for rhein(r=0.999 9),0.130-1.300 μg for emodin(r=0.999 9),0.150-1.500 μg for chrysophanol(r=0.999 9)and 0.074-0.740 μg for physcion(r=0.999 9),respectively. The RSDs of precision,stability and repeatability tests were no more than 1.96%. The av-erage recoveries were respectively 100.92%(RSD=2.80%,n=9),97.39%(RSD=1.39%,n=9),99.29%(RSD=1.81%,n=9), 100.73%(RSD=2.60%,n=9)and 99.81%(RSD=2.06%,n=9). CONCLUSIONS:The method is simple,accurate and specific, and can used for the content control of 5 constituents in Sanhuang drop pills.