Optimization of the Extraction Technology of Saponins from Paris polyphylla with Central Composite De-sign-response Surface Method
10.6039/j.issn.1001-0408.2015.28.26
- VernacularTitle:星点设计-效应面法优化重楼皂苷类成分的提取工艺Δ
- Author:
Jianfeng JU
;
Zongmin ZHU
;
Zhongke DU
- Publication Type:Journal Article
- Keywords:
Central composite design;
Response surface method;
Paridis polyphylla;
Paris saponinⅠ;
Paris saponin Ⅱ;
Pari-dis total saponin;
Extraction technology
- From:
China Pharmacy
2015;(28):3967-3969
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To optimize extraction technology of saponins from Paris polyphylla. METHODS:Using paris sapo-nin Ⅰ,paris saponin Ⅱand Paridis total saponin as dependent variables,using ethanol volume fraction,extraction time and sol-vent amount as independent variables,through multiple linear regression and binomial fitting,the extraction technology was opti-mized with response surface method and predicted. RESULTS:The optimized extraction technology of saponins from P. polyphylla was as follows as 10-fold of 80% ethanol,2 times reflux extraction,100 min each time. Under the extraction technology,the ex-traction rates of paris saponinⅠwere 85.4%,82.7% and 87.1%;those of paris saponinⅡwere 85.9%,81.3% and 83.6%;and those of Paridis total saponin were 89.5%,92.1% and 90.3%(all RSD<2.0%). Measured value was 0.964 9,predicted value was 0.986 0 and deviation rate was 2.14%. CONCLUSIONS:The central composite design-response surface method is simple and reliable for the optimization of extraction technology of saponins from P. polyphylla.
