Optimization of Water-extraction Technology of Compound Kuhuang Formulation by Orthogonal Test
10.6039/j.issn.1001-0408.2015.28.27
- VernacularTitle:正交试验法优选复方苦黄方的水提取工艺
- Author:
Zhe ZHANG
;
Mingchun LI
;
Yongjian SHI
;
Liyan ZHAO
;
Yanqin CHENG
- Publication Type:Journal Article
- Keywords:
Compound kuhuang formulation;
Extraction technology;
Orthogonal test;
Matrine;
Oxymatrine;
Prim-O-glucosyl-cimifugin;
5-O-methylvisamminol;
Berberine hydrochloride
- From:
China Pharmacy
2015;(28):3970-3973
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To optimize the water-extraction technology of Compound kuhuang formulation. METHODS:The wa-ter-extraction technology of Compound kuhuang formulation was optimized by orthogonal design,with the total contents of matrine and oxymatrine,the total content of prim-O-glucosylcimifugin and 5-O-methylvisamminol,the content of berberine hydrochloride and extract yield,and with the extraction time,amount of water(3 reflux extractions)and soaking time as the factors;and verifi-cation tests were conducted. RESULTS:The extraction time had significant effect on comprehensive evaluation value (P<0.05). The optimal water-extraction technology was as follows as extraction time of 120 min,water amount of 8,6,and 6 times as the amount of herbs,and soaking time of 20 min. In verification tests,the average total content of matrine and oxymatrine was 3.152 6 mg/g(RSD=1.03%,n=3),that of prim-O-glucosylcimifugin and 5-O-methylvisamminol was 4.977 2 mg/g(RSD=2.27%,n=3),the average content of berberine hydrochloride was 3.345 0 mg/g (RSD=1.19%,n=3) and the average extract yield was 49.23%(RSD=2.43%,n=3). CONCLUSIONS:The optimal water-extraction technology of Compound kuhuang formulation is stable and feasible.
