Inhibitory Effect and Its Mechanism of Dihydrotanshinone on Human Lung Cancer GLC-82 Cell
10.6039/j.issn.1001-0408.2015.34.08
- VernacularTitle:二氢丹参酮对人肺癌GLC-82细胞的抑制作用及其机制研究
- Author:
Bei SUN
;
Yintao YE
;
Dong WANG
;
Junqiang QIAN
;
Jianshi LOU
- Publication Type:Journal Article
- Keywords:
Human lung cancer GLC-82 cell;
Dihydrotanshinone;
Cell apoptosis;
Caspase-3 protein
- From:
China Pharmacy
2015;(34):4775-4778
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the inhibitory effect of dihydrotanshinone(DTS)on human lung cancer GLC-82 cell and its mechanism. METHODS:After treated with 0(blank control),5,10,20,40,80 and 100 μg/ml DTS for 24 and 48 h,MTT as-say was used to measure the inhibition rates and IC50 of cells;cell apoptosis was detected by flow cytometry after treated with 17.85 μg/ml DTS for 12,24 and 48 h to calculate apoptotic rate;Western blot was used to detect the protein expressions of Bcl-2, Bax and Caspase-3. RESULTS:Compared with blank control group,different concentrations of DTS inhibited the proliferation of cells;24 and 48 h maximal inhibition rate were 54.48% and 64.95%,respectively;IC50 were 62.36 and 33.94 μg/ml. DTS could induce cell apoptosis in positive time dependent manner,and the range of inhibition rate was 5.6%-29.6%;Western blot showed DTS could down-regulate the expression of Bcl-2 protein and up-regulate the expression of Caspase-3 protein (P<0.01 or P<0.05). CONCLUSIONS:DTS have significant inhibitory effect on GLC-82 cells and also induce cell apoptosis,by a possible mech-anism of down-regulating the expression of Bcl-2 protein and up-regulating the expression of Caspase-3 protein.
