Up-regulation of leptin in adipocytes exposed to high glucose and its effect in peritoneal angiogenesis
10.3760/cma.j.issn.1001-7097.2016.09.006
- VernacularTitle:高糖诱导的脂肪细胞瘦素高表达在腹膜血管新生中的作用
- Author:
Ran JING
;
Jiangzi YUAN
;
Xinghua SHAO
;
Wei FANG
;
Zhaohui NI
- Publication Type:Journal Article
- Keywords:
Adipocytes;
Neovascularization,Pathologic;
Leptin;
Glucose;
Peritoneal dialysis
- From:
Chinese Journal of Nephrology
2016;32(9):673-677
- CountryChina
- Language:Chinese
-
Abstract:
Objective By simulating a high-glucose condition of peritoneal dialysis (PD)fluid,to explore the effect of high glucose on the expression of leptin and its relationship with peritoneal angiogenesis.Methods Adipocytes differentiated from 3T3-L1 were divided into high glucose group (139 mmol/l glucose) and high mannitol group.Leptin levels in supernatant collected at 0 h,12 h,24 h and 48 h were measured by ELISA.Endothelial cells (ECs) were respectively cultured with normal glouse,high glucose,high mannitol condition,supernatants of adipocyte induced by normal glouse,high glucose and high mannitol,high glucose supernatants+leptin antibody,and high mannitol supernatants + leptin antibody.Tubular structure formation and migration of ECs were detected.Results Adipocytes exposed to high glucose for 48 h produced more leptin as compared with control group,high mannitol group,12 h-high glucose group and 24 h-high glucose group (all P ＜ 0.05).Compared with ECs in normal group,ECs in high glucose had less tubular structure formation and increased migration (all P ＜ 0.01).Compared with those of ECs in high glucose,the tubular structure formation and the migration of ECs in adipocyte supernatants induced by high glucose had increased (all P ＜ 0.01),and these effects were reduced by leptin antibody (all P ＜ 0.01).Conclusion There is an up-regulation of leptin in adipocytes exposed to high glucose,which may be an alternative way to prevent peritoneal angiogenesis.
