Study on the Preparation of Euphol Reference Substance in Euphorbia pekinensis
10.6039/j.issn.1001-0408.2015.27.31
- VernacularTitle:京大戟中大戟二烯醇对照品的制备研究
- Author:
Xiuyun GE
;
Lili SUN
;
Lelin ZHANG
- Publication Type:Journal Article
- Keywords:
Euphorbia pekinensis;
Euphol;
Reference substance;
Preparation
- From:
China Pharmacy
2015;(27):3831-3833
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a preparative separation method for euphol reference substance in Euphorbia pekinensis. METHODS:Parts of petroleum ether extraction from E. pekinensis ethanol extract were separated by silica gel column chromatogra-phy with petroleum ether-ethyl acetate(95∶5-70∶30,V/V)by gradient elution. The enriched fractions of euphol were collected. With the methanol repeated recrystallization,nuclear magnetic resonance spectroscopy(NMR)method,mass spectrometry(MS)meth-od and other spectroscopic methods were used to identify the chemical structures,and thin layer chromatography (TLC),UV, HPLC-UV,HPLC-MS were combined to detect the mass fraction. RESULTS:The mass fraction of euphol reference substance sepa-rated from E. pekinensis was>99%. CONCLUSIONS:The reference substance of euphol acquired by this method meet the relative requirements of the chemical reference substance in the content dertermination of TCM new drug quality standard. It provides chemi-cal reference substance for the quality control of E. pekinensis and prescription preparations containing E. pekinensis and the basic research of effective substances.
