Residual Determination of 7 Organic Solvents in PicrosideⅡRaw Material by Head-space GC
10.6039/j.issn.1001-0408.2015.27.41
- VernacularTitle:顶空气相色谱法测定胡黄连苷Ⅱ原料药中7种有机溶剂残留量
- Author:
Bin WANG
;
Lu ZHENG
;
Zhongqing YAO
;
Peng LI
;
Ming XUE
;
Weiwei CHEN
- Publication Type:Journal Article
- Keywords:
Head-space GC;
PicrosideⅡ;
Organic solvents;
Residual determination
- From:
China Pharmacy
2015;(27):3859-3861
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the method for the residual determination of 7 organic solvents in picrosideⅡraw materi-als. METHODS:Head-space GC was performed on the capillary column of 6% cyanopropyl phenyl-94% dimethyl polysiloxane (DB-624) by temperature programming,the temperature of injector was 200 ℃,temperature of flame ionization detector was 250 ℃,the flow rate of N2 was 35 ml/min,and split ration was 10∶1,headspace sampling was adopted with the volume of 1 ml, the heating temperature of headspace sampling was 85 ℃,heating time was 45 min. RESULTS:The good linear relationship of methanol,ethanol,ethylacetate,methylbenzene,benzene,phenylethylene and divinglbenzene had been obtained(r=0.999 6-0.999 9);RSDs of precision stability test were less than 3%;average recoveries was in the range of 78.0%-104.9%(RSDs were 0.65%-2.47%,n=6)respectively. CONCLUSIONS:The method is specific,rapid,simple and accurate,and can be used for the determination of residual organic solvents in picrosideⅡraw materials.
