MicroRNA-155 expression and clinical value in hepatocellular carcinoma
10.3760/cma.j.issn.1007-8118.2016.10.010
- VernacularTitle:MicroRNA-155在肝癌组织及细胞系中的表达及其临床意义
- Author:
Li ZHANG
;
Di ZHANG
;
Junhui LI
;
Wei WANG
;
Xuejun SUN
;
Chuantao CHENG
;
Xi CHEN
- Publication Type:Journal Article
- Keywords:
MicroRNA-155;
Hepatocellular carcinoma;
Cell cycle;
Cell proliferation;
Apoptosis
- From:
Chinese Journal of Hepatobiliary Surgery
2016;22(10):682-686
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the microRNA-155 (miR-155) expression in hepatocellular carcinoma (HCC),and to assess its correlation with clinicopathological parameters and its prognostic significance.Methods MiR-155 expression was detected in specimens of HCC and its adjacent non-tumorous liver tissues in 124 HCC patients by real-time PCR.The expression was also detected in different HCC cell lines.The Kaplan-Meier Curve was used to analyze survival of HCC patients.Gain-and loss-of-function studies were used to determine whether miR-155 impact cell cycle,cell proliferation and apoptosis of HCC cells.Results Expression of miR-155 in HCC tissues was significantly elevated when compared with that in the adjacent non-tumor liver tissues (P ＜ 0.05).MiR-155 expression was significantly higher in different HCC cell lines (P ＜ 0.05) than that in normal liver cells.MiR-155 overexpression was significantly correlated with large tumor size (P ＜ 0.05),high histological grade (P ＜ 0.05) and advanced tumor stage (P ＜ 0.05).Patients with high miR-155 expression had significantly decreased overall survival (P ＜ 0.05) and disease-free survival (P ＜ 0.05).Upregnlation of miR-155 promoted cell cycle transition from G1 to S phase (P ＜ 0.05),increased cell proliferation (P ＜ 0.05) and apoptosis resistance (P ＜ 0.05) in Hep3B cells.Downregulation of miR-155 resulted in G1 arrest,apoptosis promotion and proliferation reduction in SMMC-7721 cells (P ＜ 0.05).Conclusion MiR-155 can be used as a prognostic marker for HCC patients,and target therapy on miR-155 can be used as a potential option to prevent HCC progression.
