Blood type conversion in human liver-an experimental study on α-galactosidase
10.3760/cma.j.issn.1007-8118.2016.10.008
- VernacularTitle:α-半乳糖苷酶转换人体肝脏血型的实验研究
- Author:
Qing TIAN
;
Jianjun ZHANG
;
Yu XING
;
Shipeng LI
;
Yuliang WANG
- Publication Type:Journal Article
- Keywords:
α-galactosidase;
Human body;
Liver;
Blood type conversion
- From:
Chinese Journal of Hepatobiliary Surgery
2016;22(10):677-681
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study blood type B antigen elimination with α-galactosidase in human liver tissue,and discuss the feasibility of blood type conversion in human liver.Methods The liver specimens from patients with blood type B in liver transplantation were collected,and an in vitro liver perfusion model was established.The in vitro livers were perfused with UW solution +/-α-galactosidase.The effect of enzyme in B antigen of human liver were analyzed by immunofluorescence.Results With UW solution containing α-galactosidase to perfuse the in vitro livers,immunohistochemistry showed the level of blood type B antigen in liver was significantly reduced after hypothermic perfusion and preservation.The B antigen level in 1 h perfusion was reduced to approximate 58％ of this figure prior to perfusion,in 2 h was 10％,and in 4 h was 4％.Among the different intervals,the blood group antigen levels showed significant differences (P ＜ 0.05).In the control group,the blood group antigen levels showed no obvious change on statistical analysis.Conclusions α-galactosidase was effective to clear blood type B antigen in isolated liver tissue.In the experimental group,Although the B antigen did not fall to a undetectable level,liver blood type conversion from B→O remains a promising potential which has been meaningful for related researches on blood type conversion of human organs.
