Variable number tandem repeats diversity of Mycobacterium tuberculosis isolates
10.3760/cma.j.issn.1000-6680.2016.08.008
- VernacularTitle:结核分枝杆菌可变数目串联重复序列基因型成簇特征分析
- Author:
Dan LUO
;
Lan LAN
;
Jinming ZHAO
;
Yingkun ZHANG
;
Rushu LAN
- Publication Type:Journal Article
- Keywords:
Mycobacterium tuberculosis;
Variable number tandem repeats;
Beijing family;
Cluster
- From:
Chinese Journal of Infectious Diseases
2016;34(8):490-493
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the genetic diversity of variable number tandem repeats (VNTR) of Mycobacterium tuberculosis (M,ib) isolates and their impact factors on transmission.Methods A total of 1 310 patients with smear-positive pulmonary tuberculosis registered during 2013 from 30 counties of drug-resistance monitoring sites in Guangxi were enrolled.The VNTR genotyping technique was used to identify and analyze M.tb genotypes.Count data was analyzed by Chi-square test using descriptive statistical method,unconditioned Logistic regression was used to analyze the characteristics of gene clusters.Results A total of 1 310 strains of M.tb were classified into 964 genotypes,of which 779 strains were classified into a single gene cluster,531 strains were classified into 185 clusters.Each cluster contained 2 to 40 strains (mean 6.6).Patients aged between 41 and 60 years old had a highest gene cluster proportion (45.5%).Seven hundred and forty-six strains were Beijing genotype,while 564 were nonBeijing genotype,among which 7.4% was original Beijing genotype.The estimated recent infection (cluster rate) was 26.41%.The cluster rate was higher in sensitive strains (24.40%) than in drugresistant strains (9.55 %,x2 =23.621,P =0.000) and multi-drug resistant (MDR) strains (8.97 %,x2=-9.675,P=0.002),and that was higher in Beijing genotype (28.69%) than Non-Beijing genotype (23.40%,x2 =4.610,P=0.032).The clusters proportion in sensitive strain (37.43%) was higher than drug-resistant strains (14.09%) and MDR strains (15.38%),and that was higher in Beijing genotype (40.88%) than Non-Beijing genotype (37.77%).Conclusions M.tb genetic polymorphisms display highly diversity in Guangxi region.Sensitive strains and Beijing genotype strains are the main epidemic strains.