Confirm fusion cell line by STR technology of parentage identification

VernacularTitle:短串联重复序列亲子鉴定技术用于细胞融合的检测
Author: Yonglin YANG ; Yuting LU ; Jie CAI ; Jiling XU ; Qiang FU ; Xubing CAI
Publication Type:Journal Article
Keywords: Tandem repeat sequences; Blood grouping and crossmatching; Parent-child relations/LJ; Cell fusion
From: Journal of Chinese Physician 2016;18(9):1332-1335
CountryChina
Language:Chinese
Abstract: Objective To screen and confirm cell fusion by DNA technology of parentage identification based on detecting of short tandem repeats.Methods With 20％ polyethylene glycol (PEG)-6000,human myeloma cell lines and health individual peripheral blood mononuclear cell were fused.Then selected by hypoxantin,aminopterin,thymidin (HAT) medium,and fusion cell were sub-cloned.Morphology of fusion cells was checked by regular microscope.Concentration of DNA was compared to parental cells.Allele genes,identified by short tandem repeats,of fusion cell line were sequenced and compared with each other.Results The fused cells from myeloma cell line and peripheral blood mononuclear cell (PBMC) were slightly larger than primary cells,and the proliferation cycle was not changed significantly.DNA concentration of the fused cell DNA was increased by two times.Sequences of short tandem repeats (STR) showed that the fused cell included all original genetic materials of parent cells.Conclusions DNA technology of parentage identification is a convenient and reliable method to screen and confirm fused cell.