Karyotyping analysis on umbilical vein cord blood lymphocytes in middle-late pregnant fetus
10.3760/cma.j.issn.1008-1372.2016.08.007
- VernacularTitle:脐带血淋巴细胞染色体核型分析在孕中晚期胎儿染色体异常诊断中的作用研究
- Author:
Chang ZOU
;
Xin ZHAO
;
Xiuhua LIN
;
Huiyan HE
;
Zhuojian LIANG
;
Linhua LIN
;
Yong DAI
- Publication Type:Journal Article
- Keywords:
Fetal blood/CY;
Karyotyping;
Chromosome aberrations;
Congenital,hereditary,and neonatal diseases and abnormalities/GE;
Prenatal diagnosis;
Lymphocytes
- From:
Journal of Chinese Physician
2016;18(8):1148-1151
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the significances of karyotyping analysis on umbilical cord vein blood lymphocytes in the diagnosis of abnormal karyotypes in middle to late period of pregnant fetus.Methods A volume (0.5 ～ 1 ml) of umbilical cord vein blood was extracted from pregnant women in third trimester pregnancy with prenatal detection indications,and collected in sterilized anticoagulant tube.Lymphocytes were cultured and collected for karyotyping analysis after fixed and dropped on slides.Data were analyzed statistically.Results Lymphocytes were cultured successfully in 1 211 cases out of total 1 213 cases collected.Totally 142 abnormal karyotypes were found,which includes 81 cases (detection rate 6.68 ％) of non-heteromorphic abnormal chromosomes and 61 cases (detection rate 5.03％) of heteromorphic chromosomes.Among these abnormal karyotypes,50 cases (accounting for 35.21％ in total abnormal cases) of aneuploidy include 4 cases of chimerical karyotype.Structural abnormalities were found in 31 cases (accounting for 21.83％ in total abnormal cases) samples including 11 cases of translocations,17 cases of inversion and 3 cases of deletion.Conclusions Based on our findings,karyotyping analysis on umbilical cord vein blood lymphocytes could be an effective method for detect abnormal karyotypes in middle to late period of pregnant fetus and played an important role in prenatal diagnosis.
