Identification and genetic characteristics of a Brucella canis strain isolated in Hebei Province

VernacularTitle:河北省1株犬种布鲁菌分离株的鉴定及遗传特征分析
Author: Zhenyu QIAN ; Xiaoli LIU ; Yinqi SUN ; Zhaoyi JIA ; Baohua HE ; Xia JIANG
Publication Type:Journal Article
Keywords: Brucella canis; Multiplex polymerase chain reaction; Multiple-locus variable-number tandem-repeat analysis; Genetic characteristics
From: Chinese Journal of Endemiology 2016;35(10):717-720
CountryChina
Language:Chinese
Abstract: Objective A suspected strain of Brucella canis isolated in Hebei Province in 2015 was identified and its genetic characteristics were analyzed.Methods After conventional identification of the bacteria strain,multiplex polymerase chain reaction (M-PCR) and multiple-locus variable-number tandem-repeat analysis (MLVA) were conducted to determine its species and genetic characteristics.Results The strain was identified as Brucella canis by conventional subtyping and M-PCR.Using Panel 1,the strain was genotype 3,using Panel 2A,the strain was genotype 28.It was closely clustered with Brucella canis,and differences of repeated numbers at variablenumber tandem-repeat (VNTR) loci Bruce04,Bruce07,Bruce09 and Bruce16 were also displayed.Conclusions The conventional subtyping and molecular methods can improve the stability and accuracy of the identification.Genetic characteristics of the strain is closely related to that of Brucella canis.